General Information

Development

TIM3, also known as HAVCR2, is a cell surface protein of the TIM family expressed on Th1, Tc1, Th17, regulatory T cells, and innate immune cells (dendritic cells, NK cells, and monocytes). Its ligands include galectin-9, PtdSer, HMGB1, and CEA1. TIM3 serves as an immune checkpoint, along with other inhibitory receptors including programmed cell death protein 1 (PD-1) and lymphocyte-activation gene 3 protein (LAG3), mediates CD8⁺ T cell exhaustion. It can be used for Tim3 humanized antibody-related efficacy or drug screening experiments.

We used the CRISPR-Cas9 assisted method to replace the extracellular domain of mouse Tim3 with human sequences. In this way, in the humanized TIM3 mice, humanized TIM3 antibody inhibitors can be recognized and exert biological functions, and can be used for related efficacy or drug screening experiments.

Fig 1. The construction strategy of hTIM3 mouse

hTIM3 Mice Applications

1. hTIM3 humanized antibody related efficacy or drug screening experiments

2. Research on immunomodulation targeting TIM3 for anti-tumor, anti-infection, anti-autoimmune diseases and organ transplantation survival

Reference

1. Cao E, Zang X, Ramagopal UA, et al. T cell immunoglobulin mucin-3 crystal structure reveals a galectin-9-independent ligand-binding surface. Immunity. 2007, 26(3): 311–321.