General Information

Development

The CAG-tdTomato sequence was targeted into the Rosa26 locus to obtain the founder mice expressing red fluorescence throughout the body. The founder mice were mated with wild-type C57BL/6 mice to obtain heterozygous mice, and interbreeding of heterozygous mice resulted in homozygous mice.

This KI (knock-in) mouse line with tdTomato cDNA, under the control of the CAG promoter in Rosa26, causes all tissues except red blood cells and hair to fluoresce red under excitation light.

C57BL/6-tdTomato Mice Applications

1. In vivo cell tracking and labeling experiments.

2. Cells isolated from tdTomato red fluorescent mice may be easier to culture than primary cells from wild-type mice transfected with EGFP plasmids.

3. Tissue engineering and regenerative medicine research, which can be used to study the survival, integration, and function of transplanted cells or tissues.

Reference

1. Madisen L, Zwingman TA, Sunkin SM, et al. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci. 2010, 13(1):133-140.