General information

Development

The heterozygous mice with the Rosa-CAG-LSL-tdTomato-WPRE conditional allele are fertile. A loxP-flanked STOP cassette on one side prevents the transcription of the downstream red fluorescent protein variant (tdTomato). Since this CAG promoter-driven expression cassette is targeted to insert at the Gt(ROSA)26Sor locus, the expression of tdTomato depends on the tissue expressing the Cre recombinase. In the progeny of mice expressing the Cre recombinase, the STOP cassette is excised in the tissues expressing Cre, leading to the expression of tdTomato.

ROSA26-CAG-LSL-tdTomato Mice Applications

1. As a Cre reporter line

2. In Cre-mediated recombination after expression of the red fluorescent protein variant tdTomato, which induces red fluorescent protein expression at specific times and locations.

Reference

1. Madisen L, Zwingman TA, Sunkin SM, et al. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci. 2010, 13(1):133 -140.