CRISPR/Cas9 can target specific sequences of the genome to produce double-stranded DNA breaks, triggering non-homologous end joining. It can be used for gene knockout, small fragment gene knock-in, and conditional gene knockout, etc.
（1）Technical Process:
Vector Construction → Design and Preparation of gRNA → Microinjection → Detection of Born Mice → Obtaining Positive Mice
（2）Technical Advantages:
1. High-efficiency gene knockout, dual gRNA strategy can delete large fragments of DNA, from tens of bp to Mb, almost without length limitation.
2. Gene knock-in is heavily dependent on fragment length, and the efficiency drops sharply above 1kb.
3. High efficiency for small-scale gene editing such as point mutations.
animalmodel@vital-bj.com
+8610-84928167