Basic Information

Strain Establishment

In the severely immunodeficient mice NPG, the mouse Fap gene-specific sgRNA (single-guide RNA) was used to guide Cas9 nuclease to cut the genome, causing DSBs (Double-Stranded Breaks). Through the NHEJ (Non-homologous end Joining) repair pathway, the two cuts were directly ligated, resulting in a frameshift mutation, which achieved the goal of knocking out the Fap gene. Mice of this strain lack T, B, and NK cells, and have a functional loss of Fap.

Cancer-Associated Fibroblasts (CAFs) are closely involved in the recruitment and regulation of the immune system within the malignant microenvironment and can promote tumor immune evasion. FAP is highly expressed on CAFs in various cancers. This strain can be used to evaluate the efficacy of chimeric antigen receptor (CAR) T cells, DNA vaccines, and anti-FAP antibody targeting and depletion of fibroblast activation protein-positive (FAP+) fibroblasts.