General information

Development

CSF1 can induce hematopoietic stem cells to regulate macrophage maturation, survival, adhesion and motility. To this end, Vitonda used a transgenic method to amplify a 1.2Kb fragment upstream of the ATG of mouse CSF1 (including promoter), ligated it with the CDS of human CSF1, inserted it into the pCDNA3.1 vector, constructed the pCDNA3.1-Csf1p-hCSF1 expression vector, enzymatically isolated the fragment of the expression vector (as shown in the figure below), and injected the fragment into the NPG mice's prokaryotes of NPG mice. Positive mice with appropriate expression were screened in the obtained progeny, and the commercialized hCSF1-NPG mouse line was established by inbreeding in accordance with inbreeding in an isolator.

hCSF1-NPG mice, expressing appropriate levels of human CSF1 protein, together with NPG mice expressing human GM-CSF and IL3 cytokines, develop stable and extensive myeloid and lymphoid cell differentiation in peripheral blood, bone marrow, thymus, and spleen, as well as in non-lymphoidal tissues including lungs and livers, 6-8 weeks after transplantation of human CD34+ hematopoietic stem cells. In the blood and tissues, granulocyte differentiation (basophils, neutrophils, and mast cells), antigen-presenting cell differentiation (dendritic cells and macrophages), and regulatory T-cell populations were detected. This mouse has more precise applications in humanization, cancer therapy, human allergic reaction models, infectious diseases, immunology and regenerative medicine research.

Fig 1. Schematic diagram of hCSF1-NPG construction

NPG-hCSF1 Mice Applications

1. Humanization and cancer treatment

2. Human allergic reaction models, immunity, hematopoietic transplantation reconstruction models

3. Regenerative medicine

4. Infectious diseases

Reference

1. Rathinam C, Poueymirou WT, Rojas J, et al. Efficient differentiation and function of human macrophages in humanized CSF-1 mice. Blood. 2011, 118(11):3119-3128.

2. Wunderlich M, Chou FS, Link KA, et al. AML xenograft efficiency is significantly improved in NOD/SCID-IL2RG mice constitutively expressing human SCF, GM-CSF and IL-3. Leukemia. 2010, 24(10):1785-1788.

3. Ito R, Takahashi T, Katano I, et al. Establishment of a human allergy model using human IL-3/GM-CSF-transgenic NOG mice. J Immunol. 2013, 191(6):2890-2899.