General information

Development

A gene fragment containing the human interleukin-7 (hIL-7) encoding sequence was microinjected into the fertilized eggs of NPG mice. After genotyping, F0 generation positive mice were obtained. These F0 generation positive mice were bred with NPG mice to produce F1 generation mice. By detecting the expression levels of hIL-7 in the offspring from the F1 generation positive mice, the desired strain was acquired.

Genotype identification information

Fig1. Genotyping of NPG-hIL7

Phenotype

1. Protein expression analysis

Fig2. Human IL7 expression analysis in heterozygous NPG-hIL7 mice by ELISA. 

Serum were collected from heterozygous NPG-hIL7 mice, and analyzed by ELISA with human IL7 ELISA kit. hIL-7 expression was approximately 423 pg/mL in heterozygous NPG-hIL7.

NPG-hIL7 Mice Applications

1.Immunotherapy

2.Tumor research

3. Inoculation of human tumors for screening of relevant drugs

4. Preparation of humanized mouse models of the immune system, such as those obtained by inoculation with PBMC, PBNK and HSC

5. Research on human hematopoietic system and immune system

6. Human cell and tissue transplantation