Pronuclear microinjection, 8-cell injection, blastocyst injection, Piezo-ICSI injection, non-Piezo-ICSI injection, tetraploid blastocyst injection, aggregation chimeras of sandwich combining ~20 ES Cells and two tetraploid embryos, embryo transfer into the oviduct, embryo transfer into the uterus and various other microsurgical services can be provided by Vitalstar Biotechnology.

At the same time, different rat & mouse strains, different embryo stages and different doses (or quantities) can be injected according to customer requirements, and specific cells, viruses, dyes or other biological materials can also be injected.

With more than 20 years of experience, the Vitalstar Biotechnology provides key services including:

• Pronuclear microinjection

• Cytoplasmic injection

• ICSI injection

• 8-cell injection

• Blastocyst injection

• Aggregation chimeras of sandwich combining ~20 ES Cells and two tetraploid embryos

• Custom micromanipulation

Microinjection services of Vitalstar Biotechnology have delivered thousands of chimeric mice, thousands of gene-edited mice and in vitro fertilized mouse & rat strains. Services for scientific research clients, participating in the microinjection services section, are published in the most influential journals such as Science, Cell,Cell Stem Cell and Cell Res.

1. Articles published using this technology

（1）Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency

（2）A XEN-like State Bridges Somatic Cells to Pluripotency during Chemical Reprogramming

（3）Derivation of totipotent-like stem cells with blastocyst-like structure forming potential

（4）Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg^-/-mice

（5）Mouse totipotent stem cells captured and maintained through spliceosomal repression