General Information

Development

LAG3 (Lymphocyte-activation gene 3), also known as CD223, is a cell surface protein molecule with multiple biological functions. LAG3 is expressed on activated T cells, B cells, NK cells, and plasmacytoid dendritic cells, and its main ligand is the MHC class II molecule. This protein, similar to CTLA-4 and PD-1, negatively regulates T cell proliferation, activation, and homeostasis, and has been reported to play a role in Treg suppressive function. It can also be used for LAG3 humanized antibody-related efficacy or drug screening experiments.

We used the CRISPR-Cas9 assisted method to replace the extracellular domain of mouse LAG3 with human sequences. In this way, in the humanized LAG3 mice, human LAG3 antibody inhibitors can be recognized and exert biological functions, which can be used for related efficacy or drug screening experiments

Fig 1. Construction strategy of hLAG3 mice

hLAG3 Mice Applications

1. Pharmacodynamic or drug screening experiments related to LAG3 humanized antibody

2.Research on immunomodulation targeting LAG3 for anti-tumor, anti-infection, anti-autoimmune diseases and organ transplantation survival

Reference

1. Ruffo E, Wu RC, Bruno TC, et al. Lymphocyte-activation gene 3 (LAG3): The next immune checkpoint receptor. Semin Immunol. 2019, 42:101305.