General Information

Development

In C57 mice, mouse Alms1 gene-specific sgRNA (single-guide RNA) was utilized to mediate Cas9 nuclease cleavage of the genome, resulting in DSBs (Double-Stranded Breaks), which were directly joined through the NHEJ (Non-homologous end Joining) repair pathway, resulting in a shift mutation. directly connecting the two cuts through the NHEJ (Non-homologous end Joining) repair pathway, resulting in a shifted mutation to achieve knockdown of the Alms1 gene. An Alms1 mutant mouse model with a combined phenotype of obesity, diabetes and NAFLD was developed.

When fed regular chow, this strain develops obesity, moderate hyperglycemia, and progressively worsens hyperlipidemia as well as liver damage with age. Feeding a Western diet accelerated the process of metabolic disorders and liver damage.

Alms1 ko Mice Applications

1. Evaluation of drug efficacy in metabolic diseases such as obesity, diabetes mellitus and metabolic dysfunction-associated fatty liver diseases

2. Research on the mechanism of metabolic diseases such as obesity, diabetes mellitus and metabolic dysfunction-related fatty liver disease

3. Evaluation of the efficacy of complications caused by metabolic diseases