Diabetes is a chronic metabolic disease characterized by high blood sugar content, mainly due to insufficient insulin secretion (hypoislet function) or the body's use of insulin efficiency is reduced (insulin resistance), difficult to cure, generally require lifelong medication. Diabetes is mainly divided into type I diabetes and type II diabetes and other special types of diabetes, of which type II diabetes in diabetes patients accounted for about 90%. Triggers generally include strong autoimmune responses (type I), obesity, bad eating habits, lack of exercise, and genetic factors (type II).

Through gene editing technology and diet-induced method, Vitalstar has constructed the corresponding diabetes model. At the same time, it is equipped with a series of sampling and testing equipment, which can provide one-stop pre-clinical research services for diabetes model, for the pathogenesis research and treatment drug screening evaluation of type I and type II diabetes.

(1) HFD+STZ induced MASH-HCC model and its drug efficacy studies

1. Experimental scheme

C57BL/6J male mice were given a single subcutaneous injection of 200 ug STZ (Sigma) 2 days after birth and fed a diet of HFD32 from 4 to 20 weeks of age to induce NASH-HCC. Control mice (C57BL/6J) were fed normal diet without treatment.

2. Experimental data

Body weight, blood sugar level, blood lipid level (cholesterol, triglyceride, etc.), liver function (ALT, AST, etc.) were observed regularly. Liver histology (HE, oil red O staining) was used to observe steatosis, inflammation and fibrosis (TGF-β, α-SMA, collagen, etc.).

(2) STZ induced type I diabetes model

1. Experimental scheme

C57BL/6J was intraperitoneally injected with 50 mg STZ/kg/ body weight for 5 consecutive days, and blood glucose was measured 14 to 16 days after injection. KM male rats were given a single intraperitoneal injection (1 day) of 200 mg STZ/kg/ body weight and blood glucose was measured 3 days after injection.

2. Experimental data

Body weight data, fasting blood glucose, serum insulin levels, glycosylated hemoglobin (HbA1c), and lipid levels (changes in total cholesterol, LDL, and HDL) were continuously monitored. Statistics on the success rate of STZ-induced diabetes (from 30 mice in a single experiment, the success rate will change with each experiment, this table is for reference only).

*18 mg/dL=1mM(＞13.8mM or 250 mg/dL,indicates successful establishment of diabetes model).