Vitalstar provides a serial of immunodeficient mouse strains suitable for establishing human peripheral blood mononuclear cell (PBMC) engraftment models, such as NPG, NRG, DK-NPG, NPG-hIL15, etc., catering to different research purposes. We have also selected PBMC products from different donors for to establish PBMC-NPG mouse models and set up a PBMC stockage for research use. We can provide users with qualified human PBMC mouse models and related services.

Service Content：

1. Human PBMC transplanting to mice.

2. Analysis of human immune system reconstitution in mice (Flow Cytometry).

3. Donor selection for establishment of human PBMC mouse models.

4. Establishment of human-to-mouse Graft-versus-Host Disease (GvHD) mouse model and efficacy study of potential treatments against GvHD.

5. Establishment of mouse-to-mouse (splenocyte transplantation between different strains) GvHD models and study of the disease.

Case I：Screening of PBMC derived from different donors when transplanting into NPG mice

Figure 1.The survival rates, body weights, GvHD scores and human immune cell proportions in blood of Hu-PBMC-NPG mice transplanted with PBMCs derived from different donors

Case II：Screening of PBMC derived from different donors when transplanting into DK-NPG mice

Figure 2. The survival rates, body weights, GvHD scores and human immune cell proportions in blood of Hu-PBMC-DK-NPG mice transplanted with PBMCs  (1E7 cells/mouse) derived from different donors

Case III：Evaluation of treatment effect of human amniotic mesenchymal stem cells (hAMSCs) on a mouse model of graft-versus-host disease (GVHD) ^[1]

Figure 3. Observation of therapeutic effect of human amniotic mesenchymal stem cells (hAMSCs) on a mouse aGvHD model

Irradiated NPG mice were transplanted with human PBMCs to establish a model of acute graft-versus-host disease (aGVHD). The aGVHD mice were divided into two groups; one group mice were injected with 5×10⁵ hAMSCs via the tail vein and the other group mice were injected with PBS as control, 3 days after PBMC injection. Results showed that hAMSCs significantly improved the severity of acute GVHD in terms of weight loss and disease scoring, prevented leukocyte infiltration, and reduced pathological changes in the liver, spleen, lung, and intestine of the mice.

References：

1. Gao Y, Li W, Bu X, et al. Human Amniotic Mesenchymal Stem Cells Inhibit aGVHD by Regulating Balance of Treg and T Effector Cells. J Inflamm Res. 2021, 14:3985-3999.