The Vitalstar Flow Cytometry Platform is currently equipped with the CytoFLEX flow cytometer from BECKMAN COULTER and the FACSCanto Plus flow cytometer from BD. It offers detection and analysis based on flow cytometry to determine the proportions and numbers of various cell populations in human/mice/ect peripheral blood/spleen/tumor and other tissues, such as T cells, B cells, NK cells, monocytes, granulocytes, macrophages, and dendritic cells (DCs), to assess the composition and function of the immune system. In addition, after gene knockout, target modification, or drug induction in mice/rats, immune cells will undergo changes. By analyzing the conditions of various immune cells through flow cytometry, it is possible to identify the cell populations affected by genes or drugs, which is helpful for the functional study of genes and drugs.

The application scenarios include:

1. Immune cell clustering

2. Detection and analysis of cell surface marker expression levels and the proportion of positive cells

3. Detection and analysis of intracellular protein expression levels and the proportion of positive cells

4. Detection and analysis of cell apoptosis/cell cycle/cell proliferation

5. Detection and analysis of cytokine content (Cytometric Bead Array)

Case I: flow cytometry analysis of mouse splenocytes

Fig 1. Flow cytometry detection of T cell activation in mouse spleen/

After staining mouse splenocytes with CD45, CD3, CD4, and CD8 fluorescent antibodies, followed by fixation and permeabilization, intracellular staining is performed with IFNγ and TNFα fluorescent antibodies, and then analyzed by flow cytometry.